Natural Compounds of Ethanolic Fruit Extract of Citrullus Colocynthis Screening and Assessment of Its Antifungal Activity

Citrullus colocynthis is also known as bitter cucumber, bitter apple and colocynth. It is now classified as Citrullus colocynthis, where it used to be called Colocynthis citrullus. Medicinal plants have secondary metabolism to produce organic compounds, including tannins, alkaloids, carbohydrates, triterpenoids, steroids, flavonoid compounds, etc. These compounds possess physiological influences on human beings and antimicrobial action on a range of microbes. Based on the peak (%), the prevalent bioactive compounds were 1H-Pyrrole-2-carboxaldehyde, 2'-Hydroxy-5'-methylacetophenone, 3-Nonyn-2-ol, spiro[3H-chromene-2,1'-cyclobutane]-4-one, 4-propanoylphenyl 2,2-dimethylpropanoate, 6-Hydroxyhexanoic acid lactone, Octadecanoic acid, tetradecyl ester, 4-(3-Hydroxy-1-propenyl)-2-methoxyphenol, 4-Phthalimidobutyric acid, n-Tetradecanal, 3-Trifluoroacetoxydodecane, octahydropyrrolo[1,2-a]azocin-5(1H)-one, (R)-alpha-Hydroxy-gamma-butyrolactone, N-(4-Methoxyphenyl)-acetylacetamide, 1-Pentanol, 2,3-dimethyl, Glutaraldehyde, 3-methyl, 3,3-Diphenyldihydrofuran-2(3H)-one, Catechol diethyl ether, 1H-Indole, 2,3-dihydro-2-methyl and 9,12-Octadecadienoic acid, ethyl ester. Bioactivity of Hollyhock (Citrullus colocynthis) methanolic crude extract and ethanol fraction in inhibiting three fungi in comparison to traditional antibiotics. besides the popular antibiotics Posaconazole (PCZ) and Itraconazole (ICZ). Observed on Aspergillus flavus (18.08±0.39, 21.00±0.43, 31.09±0.52 and 25.00±0.46). Monitored on F. oxyporum (16.96 20.08, 12.00 22.08 and 19.45). Repeated versus Cladosporium herbarum concomitantly (24.58±0.45, 19.00±0.40, 34.00±0.57, and 27.95±0.50). The activity of citrullus colocynthis metabolites was very high (24.58±0.45) towards Cladosporium herbarum.