CRISPR-Cas Systems in Bacteria: A Review of Mechanisms and Applications
10.52845/CMRO/2024/7-11-2
Abstract
This review analyzes the development and various uses of the CRISPR-Cas systems in bacterial cells, emphasizing their function in adaptive immunity and their potential as effective tools in bioengineering. Consisting of arrays of CRISPR and P proteins, the CRISPR-Cas systems defend bacteria and archaea from viruses by targeting and destroying foreign genetic material sequences. Some of the literature on CRISPR systems subclasses has been reviewed, and basic stages of select bacteria practice modification over the nature and return of that genetic material are described. Class 1 and Class 2 types are particularly stressed, including Cas9 and Cpf1 proteins respectively which made a breakthrough in gene editing technologies. This paper also evaluates the recent trends in the CRISPR technology and its usage in genetic engineering, personalized medicine and diagnostics as well as the recent advances in gene editors, RNA targeting and strategically focused approaches towards antimicrobial resistance. Whether used in genetic stabilizing or cutting precise targeting, the review points out the off-target products and many other ethical issues associated with the techniques including human therapeutic applications in target genome editing. The overall aim of this article to provide guidelines to the researchers willing to employ the CRISPR-Cas systems and the genetic modification technology in developing better biotechnological products and therapeutic techniques in the future.
Keywords
- CRISPR-Cas systems
- Bacterial immunity
- Gene editing
- Cas9 protein
- Genome engineering
How to Cite
Mais E. Ahmed, Hiba Mahdi Msbah, Zinah Shamil Kamil . (2024). CRISPR-Cas Systems in Bacteria: A Review of Mechanisms and Applications. Journal of Current Medical Research and Opinion , Vol. 7 No. 11 (2024) , 3699-3713 . https://doi.org/10.52845/CMRO/2024/7-11-2