Screening of Metabolites Produced by Pathogenic Escherichia coli and Evaluation of its Antimicrobial Activity

Background: First, the term ‘metabolic processes’ refers to all the biochemical reactions which occurs in a given organism, and this is relevant for our aims and objectives as stated below: Substrate oxidations and dissimilation reaction is the chemical variation in bacterial metabolism that energy bacteria employ in the production of energy. Bacterial metabolites play basic roles in human biochemistry; they constitute an unexploited reservoir of drugs effective against a wide spectrum of diseases. In this work, the bioactive chemical compounds produced by Escherichia coli when synthesizing BCs were important for assessing the efficacy of the synthesized BCs against different bacteria.

Methods: The primary and secondary metabolites produced and referred to as the bioactive chemical compound were analyzed using gas chromatography-mass spectrometry (GC-MS) analysis. The antibacterial activity in vitro of the ethanolic extract of Escherichia coli was also determined.In E. coli GC-MS analysis the following compounds were found: objectives. It is worth to mention that substrate oxidations and dissimilation reactions are chemically diverse aspects of bacterial metabolism as a means of producing energy. Metabolites created by bacteria are essential for human survival and are also considered to be excellent drug candidates for a large number of diseases. The researchers sought to identify the effectiveness of bioactive chemical compounds that are secreted from Escherichia coli on various bacteria types.

Methods: The bioactive chemical components also referred to as secondary metabolites were determined using gas chromatography-mass spectrometry (GC-MS) practices. The above ethanolic extract of Escherichia coli was also assessed its efficacy profiles by the in vitro antibacterial assay.

Result: Following are the list of compounds: tert-Butyl 12-aminododecanoate, 1,12-Diaminododecane, Ethylidenehydroxylamine, 5,6-Diamino-2,3-dicyanopyrazine, 1 -methylpiperidine-3-Mannose, D-Ornithine, 5-Hydroxy-L-tryptophan, Glycerophosphocholine. This research compared the efficacy of two common antibiotics, RF-Rifampicin and SF-Sulfonamide, with an ethanolic extract of Escherichia coli against five different bacteria: , Enterococcus faecalis (14.54±0.11; 23.99±0.23; 17.97±0.20), Bacillus subtilis (15.12±0.21; 25.27±0.26; 19.00±0.2), Streptococcus pyogen Comparing the average activity against all the tested microorganisms, the metabolites of Escherichia coli showed highest activity against Streptococcus pyogenes (15.12±0.21).