Analysis of Clinical Klebsiella pneumoniae Isolates for Carbapenemase Genes by Molecular Method
10.52845/CMRO/2026/9-8-3
Abstract
Opportunistic pathogen Klebsiella pneumoniae is known to be resistant to many drugs, including carbapenems. Examining the genetic makeup of several carbapenemase-encoding genes in clinical K. pneumoniae isolates was the primary objective of this investigation. Tradition
al single-plex polymerase chain reaction (PCR) was employed to identify the blaNDM, blaOXA-48-like, and blaKPC genes in 35 clinical K. pneumoniae isolates. The results demonstrated that the isolates examined had an abundance of genes related to carbapenemase. The blaOXA-48 gene was detected in all 35 isolates (100%), while blaNDM was detected in 33 isolates (94.28%). In contrast, the blaKPC gene was not detected in any of the examined isolates (0%). Regarding antimicrobial resistance classification, 23 isolates (65.71%) were multidrug-resistant (MDR), 7 (20%) were extensively drug-resistant (XDR), and 14.28% were classified as non-MDR. The molecular findings demonstrated a predominance of blaOXA-48, followed by blaNDM, whereas blaKPC was absent among the studied isolates.
Keywords
- Klebsiella pneumoniae
- carbapenem resistance
- blaNDM
- blaOXA-48
- blaKPC
- Carbapenemase Genes
- Antimicrobial Resistance
How to Cite
Karrar Majid Hameed, Safa Hasan Radhi . (2026). Analysis of Clinical Klebsiella pneumoniae Isolates for Carbapenemase Genes by Molecular Method. Journal of Current Medical Research and Opinion , Vol. 9 No. 08 (2026) , 4822-4826 . https://doi.org/10.52845/CMRO/2026/9-8-3