Secretion Levels of Some Endogenous Antimicrobial Peptides and Cytokines in Type 2 Diabetes Mellitus
The article presents data on the level of secretion of endogenous antimicrobial peptides and cytokines in type 2 diabetes mellitus.
Aim. To assess the secretion levels of antimicrobial peptides (defensin and endotoxin) and cytokines (interleukin-8, tumor necrosis factor α and transforming growth factor β1) in blood plasma in type 2 diabetes mellitus.
Methods. Blood samples of 87 patients with type 2 diabetes mellitus were examined. All patients were assigned to three groups depending on blood glucose and glycosylated hemoglobin levels: compensation – 36 subjects, subcompensation – 34 subjects, decompensation – 17 patients. ELISA test was used to measure the levels of cytokines («Bender Medsystems» kits, Austria) and antimicrobial peptides (set «Hycult Biotech»).
Results. In the first group (compensation stage), defensin level was 17.6% higher than in the control group. The second group (subcompensation) showed a 4-fold increase in defensin level, while in the third group (decompensation stage) there was a 14.4-fold increase, compared to the control. Endotoxin concentration was 1.18 ± 0.05 ng/ml in the first group, 1.57 ± 0.14 ng/ml in the second group, and 18.2 ± 0.09 for the third group, where the control value was 0.33 ± 0.12 ng/ml.
Conclusion. An association was revealed between the secretion of antimicrobial peptides and cytokines. Concentration of antimicrobial peptides and cytokines reflects the stage of type 2 diabetes mellitus.